| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Loder, Kathleen L (13)
|
IADSMY |
|
37.30 |
- |
| 2 |
Reynolds, Alexes (14)
|
IANVFY |
|
38.36 |
- |
| 3 |
Thompson, Katherine G (14)
|
IADSMY |
38.28 |
38.54 |
0.26 |
| 4 |
Wheatley, Isabell (13)
|
IANVFY |
39.60 |
39.10 |
-0.50 |
| 5 |
Clayton, Karter (13)
|
SPS |
43.65 |
42.20 |
-1.45 |
| 6 |
Robben, Rylynn J (13)
|
IADSMY |
44.06 |
43.31 |
-0.75 |
| 7 |
Hodges, Adrienne V (14)
|
IADSMY |
43.83 |
43.64 |
-0.19 |
| 8 |
McDermott, Isabelle (14)
|
IANVFY |
44.69 |
43.92 |
-0.77 |
| 9 |
Megonigle, Maddi P (13)
|
IADSMY |
44.40 |
45.02 |
0.62 |
| 10 |
Phillips, Madilynn M (13)
|
IADSMY |
1:13.32 |
1:02.15 |
-11.17 |